Hoefer HB1000 - Instrukcja (Strona 29)

•
p20
Protocol
3
Chemiluminescence detection:
HRP-tagged, alkaline phosphatase (AP)
probes or antibody conjugates
Equipment
• Clear plastic cling-wrap or clear transparent
sheet protector.
• Hoefer ULTima Pro imaging system with cooled
CCD camera.
• Pipette
Reagents (Chemiluminescence)
• ECL ™ (or other) detection reagent 1
• ECL (or other if required) detection reagent 2
• Membrane following hybridization
Procedure
1. Mix equal volumes of detection reagents 1 and 2.
2. Pipette the mixture over the surface of the
membrane and leave at room temperature for
1 minute.
3. Follow the wash instructions provided by the kit
manufacturer. Remove the final wash and cover the
membrane in plastic wrap.
4. Image using the Hoefer ULTima Pro chemilumines-
cence imaging system.
References
Ross, J (1998). Nucleic Acid Hybridization:
Essential Techniques. John Wiley and Sons, ISBN
0-471-97125-1.
Sambrook, J (2001). Molecular Cloning: A Laboratory
Manual (Third Edition). Cold Spring Harbor Labora-
tory Press, ISBN 0-87969-577-3.
Current Protocols in Molecular Biology (1987). John
Wiley and Sons; ISBN 0-471-50338-X.