Hoefer UVC500 - Instrukcja (Strona 15)

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p6
Energy setting
The amount of energy recommended for UV
crosslinking of DNA and RNA by covalently
binding nucleic acids to Hybond
™ membranes
after Northern, Southern, slot or dot blotting
is 70,000 µJ/cm2. This value is preset at the
factory and displays as 700 on the LED display,
which only shows 4 digits. As the unit operates,
a countdown of the remaining energy to be emit-
ted is displayed on the LED.
Using the preset energy setting
1
First press the PRESET key and then the ENERGY
key. The red indicator lamp next to each of these keys
should light and the LED should display 700
(or the value last programmed).
2
Press the green START key. The UV lamps take a few
seconds to energize before the countdown value is
displayed. At the end of the exposure, the unit auto-
matically stops and beeps 5 times.
Reprogramming the preset energy setting
1
Press and hold the PRESET key until you hear a
second beep. The lamp next to the PRESET key
flashes when this key switches to program mode.
2
Press the ENERGY key. Use the numeric keypad to
enter the new value. The LED display should show the
new value.
3
Press the ENTER key.
Note: Multiply all displayed
energy settings by 100 for the
actual energy value.
UVC500 Crosslinker
user interface
The LED display shows values for
energy (multiply by 100 for actual
value in µJ/cm
2 ) and time (in
minutes and tenths of a minute.)