Operating instructions – Hoefer UVC500 Instrukcja Obsługi

Strona 14

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Operating instructions

Set up and membrane placement

1

Place the unit on a stable and level surface.

2

Plug the power cord into the receptacle at the back of
the unit and into a properly grounded electrical outlet
of the proper power rating.

3

Flip the power mains switch located under the keypad
to on (–).

Note: The instrument settings are always the values
last programmed, and are indicated by the LED
display and a lit red indicator lamp.

4

Open the door by pulling the door handle above the
window toward you.

Note: Do not obstruct or damage the photofeed-
back sensor, which is mounted on a small metal tab
protruding from the lower right side.

5

Moisten (do not wet completely) a sheet of blotting
paper with transfer buffer and place it in the center of
the compartment. Place the membrane on the paper
with the nucleic acid side facing upward.

6

Set the energy exposure (energy or time) as described
in the next section.

p5

Note: To protect the cross-
linker from corrosive buffers,
lay a piece of plastic wrap
on the compartment bottom
before placing the blotting
paper and sample.

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